Sterility:Article Title: Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis.
Article Snippet: .. N/A N/A 384-well LabCyte sterile plate (source plate for ECHO525) without clear seal - optional for low volume reactions. (steps 11 to 16) Labcyte.com PPL-0200 384-well for the real-time PCR, BioRad or for the specific qPCR instrument used. (steps 7 to 31) Bio-Rad Laboratories HSP3805 or HSP3905 Optically clear seal for 384-well plates, or according to the type of the instrument. (steps 11 to 31) Bio-Rad Laboratories MSB1001 Aluminum foil plate seal, for - 80 C to - 130 C (steps 9 to 36) Beckman Coulter 538619 Reagent Reservoirs, 25 mL, for multichannel pipettes (steps 11 to 31) VWR 8093-11 pH paper strips (step 11 to 24) Any Any Cold packets frozen at 20 C (step 11 to 24) Any Any 96-well plates that fit in any available regular thermocycler (step 32 to 36) Any Any Refrigerator/Freezer, depending on the sample normal habitat temperature range, 4 C, 20 C, 80 C (see step 1b) N/A N/A (Continued on next page) ll OPEN ACCESS STAR Protocols 3, 100968, March 18, 2022 3 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell Sorter, here described BD Influx , or similar instruments: e.g., Wolf G2, that can do FACS into plates and tubes (steps 7 to 10) BD Biosciences nanocellect choose Wolf G2 2 sheath fluid tanks and one waste tank for the BD Influx Cell Sorter (steps 7 to 10) Order specific to instrument Order specific to instrument small sonic bath, e.g., Ultrasonic Model B200 (steps 7 to 10) VWR # 100491-732 small vortex, e.g., VWR LabDancer Mini Vortexer. (steps 7 to 31) VWR # 82019-172 PCR hood or Biosafety hood with UV bulbs, any (steps 1 to 32) N/A N/A Stratagene UV Stratalinker 2400 or equivalent (steps 1 to 31) LabX or ALT Stratagene Stratalinker 2400 or ALT any microscope with fluorescent light capability and glass slides (steps 1 to 10) N/A N/A BioRad SFX384 Real-time thermocycler or similar (step 11 to 31, step 1d) Bio-Rad Laboratories 12011452 Optional: Labcyte ECHO525 automatic liquid handler – optional for low volume reactions. (step 11 to 24) Labcyte.com 001-10080 Benchtop microcentrifuge, any for 1.5–2 mL tubes. (steps 11 to 36) Stellar Scientific BS-MFUG-12 Swing bucket centrifuge for plates, ideally refrigerated. (steps 11 to 36) Any Any Any regular thermocycler for 96-well plates. (step 32 to 36) Any Any ll OPEN ACCESS Protocol ..
Real-time Polymerase Chain Reaction:Article Title: Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis.
Article Snippet: .. N/A N/A 384-well LabCyte sterile plate (source plate for ECHO525) without clear seal - optional for low volume reactions. (steps 11 to 16) Labcyte.com PPL-0200 384-well for the real-time PCR, BioRad or for the specific qPCR instrument used. (steps 7 to 31) Bio-Rad Laboratories HSP3805 or HSP3905 Optically clear seal for 384-well plates, or according to the type of the instrument. (steps 11 to 31) Bio-Rad Laboratories MSB1001 Aluminum foil plate seal, for - 80 C to - 130 C (steps 9 to 36) Beckman Coulter 538619 Reagent Reservoirs, 25 mL, for multichannel pipettes (steps 11 to 31) VWR 8093-11 pH paper strips (step 11 to 24) Any Any Cold packets frozen at 20 C (step 11 to 24) Any Any 96-well plates that fit in any available regular thermocycler (step 32 to 36) Any Any Refrigerator/Freezer, depending on the sample normal habitat temperature range, 4 C, 20 C, 80 C (see step 1b) N/A N/A (Continued on next page) ll OPEN ACCESS STAR Protocols 3, 100968, March 18, 2022 3 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell Sorter, here described BD Influx , or similar instruments: e.g., Wolf G2, that can do FACS into plates and tubes (steps 7 to 10) BD Biosciences nanocellect choose Wolf G2 2 sheath fluid tanks and one waste tank for the BD Influx Cell Sorter (steps 7 to 10) Order specific to instrument Order specific to instrument small sonic bath, e.g., Ultrasonic Model B200 (steps 7 to 10) VWR # 100491-732 small vortex, e.g., VWR LabDancer Mini Vortexer. (steps 7 to 31) VWR # 82019-172 PCR hood or Biosafety hood with UV bulbs, any (steps 1 to 32) N/A N/A Stratagene UV Stratalinker 2400 or equivalent (steps 1 to 31) LabX or ALT Stratagene Stratalinker 2400 or ALT any microscope with fluorescent light capability and glass slides (steps 1 to 10) N/A N/A BioRad SFX384 Real-time thermocycler or similar (step 11 to 31, step 1d) Bio-Rad Laboratories 12011452 Optional: Labcyte ECHO525 automatic liquid handler – optional for low volume reactions. (step 11 to 24) Labcyte.com 001-10080 Benchtop microcentrifuge, any for 1.5–2 mL tubes. (steps 11 to 36) Stellar Scientific BS-MFUG-12 Swing bucket centrifuge for plates, ideally refrigerated. (steps 11 to 36) Any Any Any regular thermocycler for 96-well plates. (step 32 to 36) Any Any ll OPEN ACCESS Protocol ..
FACS:Article Title: Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis.
Article Snippet: .. N/A N/A 384-well LabCyte sterile plate (source plate for ECHO525) without clear seal - optional for low volume reactions. (steps 11 to 16) Labcyte.com PPL-0200 384-well for the real-time PCR, BioRad or for the specific qPCR instrument used. (steps 7 to 31) Bio-Rad Laboratories HSP3805 or HSP3905 Optically clear seal for 384-well plates, or according to the type of the instrument. (steps 11 to 31) Bio-Rad Laboratories MSB1001 Aluminum foil plate seal, for - 80 C to - 130 C (steps 9 to 36) Beckman Coulter 538619 Reagent Reservoirs, 25 mL, for multichannel pipettes (steps 11 to 31) VWR 8093-11 pH paper strips (step 11 to 24) Any Any Cold packets frozen at 20 C (step 11 to 24) Any Any 96-well plates that fit in any available regular thermocycler (step 32 to 36) Any Any Refrigerator/Freezer, depending on the sample normal habitat temperature range, 4 C, 20 C, 80 C (see step 1b) N/A N/A (Continued on next page) ll OPEN ACCESS STAR Protocols 3, 100968, March 18, 2022 3 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell Sorter, here described BD Influx , or similar instruments: e.g., Wolf G2, that can do FACS into plates and tubes (steps 7 to 10) BD Biosciences nanocellect choose Wolf G2 2 sheath fluid tanks and one waste tank for the BD Influx Cell Sorter (steps 7 to 10) Order specific to instrument Order specific to instrument small sonic bath, e.g., Ultrasonic Model B200 (steps 7 to 10) VWR # 100491-732 small vortex, e.g., VWR LabDancer Mini Vortexer. (steps 7 to 31) VWR # 82019-172 PCR hood or Biosafety hood with UV bulbs, any (steps 1 to 32) N/A N/A Stratagene UV Stratalinker 2400 or equivalent (steps 1 to 31) LabX or ALT Stratagene Stratalinker 2400 or ALT any microscope with fluorescent light capability and glass slides (steps 1 to 10) N/A N/A BioRad SFX384 Real-time thermocycler or similar (step 11 to 31, step 1d) Bio-Rad Laboratories 12011452 Optional: Labcyte ECHO525 automatic liquid handler – optional for low volume reactions. (step 11 to 24) Labcyte.com 001-10080 Benchtop microcentrifuge, any for 1.5–2 mL tubes. (steps 11 to 36) Stellar Scientific BS-MFUG-12 Swing bucket centrifuge for plates, ideally refrigerated. (steps 11 to 36) Any Any Any regular thermocycler for 96-well plates. (step 32 to 36) Any Any ll OPEN ACCESS Protocol ..
Polymerase Chain Reaction:Article Title: Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis.
Article Snippet: .. N/A N/A 384-well LabCyte sterile plate (source plate for ECHO525) without clear seal - optional for low volume reactions. (steps 11 to 16) Labcyte.com PPL-0200 384-well for the real-time PCR, BioRad or for the specific qPCR instrument used. (steps 7 to 31) Bio-Rad Laboratories HSP3805 or HSP3905 Optically clear seal for 384-well plates, or according to the type of the instrument. (steps 11 to 31) Bio-Rad Laboratories MSB1001 Aluminum foil plate seal, for - 80 C to - 130 C (steps 9 to 36) Beckman Coulter 538619 Reagent Reservoirs, 25 mL, for multichannel pipettes (steps 11 to 31) VWR 8093-11 pH paper strips (step 11 to 24) Any Any Cold packets frozen at 20 C (step 11 to 24) Any Any 96-well plates that fit in any available regular thermocycler (step 32 to 36) Any Any Refrigerator/Freezer, depending on the sample normal habitat temperature range, 4 C, 20 C, 80 C (see step 1b) N/A N/A (Continued on next page) ll OPEN ACCESS STAR Protocols 3, 100968, March 18, 2022 3 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell Sorter, here described BD Influx , or similar instruments: e.g., Wolf G2, that can do FACS into plates and tubes (steps 7 to 10) BD Biosciences nanocellect choose Wolf G2 2 sheath fluid tanks and one waste tank for the BD Influx Cell Sorter (steps 7 to 10) Order specific to instrument Order specific to instrument small sonic bath, e.g., Ultrasonic Model B200 (steps 7 to 10) VWR # 100491-732 small vortex, e.g., VWR LabDancer Mini Vortexer. (steps 7 to 31) VWR # 82019-172 PCR hood or Biosafety hood with UV bulbs, any (steps 1 to 32) N/A N/A Stratagene UV Stratalinker 2400 or equivalent (steps 1 to 31) LabX or ALT Stratagene Stratalinker 2400 or ALT any microscope with fluorescent light capability and glass slides (steps 1 to 10) N/A N/A BioRad SFX384 Real-time thermocycler or similar (step 11 to 31, step 1d) Bio-Rad Laboratories 12011452 Optional: Labcyte ECHO525 automatic liquid handler – optional for low volume reactions. (step 11 to 24) Labcyte.com 001-10080 Benchtop microcentrifuge, any for 1.5–2 mL tubes. (steps 11 to 36) Stellar Scientific BS-MFUG-12 Swing bucket centrifuge for plates, ideally refrigerated. (steps 11 to 36) Any Any Any regular thermocycler for 96-well plates. (step 32 to 36) Any Any ll OPEN ACCESS Protocol ..
Microscopy:Article Title: Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis.
Article Snippet: .. N/A N/A 384-well LabCyte sterile plate (source plate for ECHO525) without clear seal - optional for low volume reactions. (steps 11 to 16) Labcyte.com PPL-0200 384-well for the real-time PCR, BioRad or for the specific qPCR instrument used. (steps 7 to 31) Bio-Rad Laboratories HSP3805 or HSP3905 Optically clear seal for 384-well plates, or according to the type of the instrument. (steps 11 to 31) Bio-Rad Laboratories MSB1001 Aluminum foil plate seal, for - 80 C to - 130 C (steps 9 to 36) Beckman Coulter 538619 Reagent Reservoirs, 25 mL, for multichannel pipettes (steps 11 to 31) VWR 8093-11 pH paper strips (step 11 to 24) Any Any Cold packets frozen at 20 C (step 11 to 24) Any Any 96-well plates that fit in any available regular thermocycler (step 32 to 36) Any Any Refrigerator/Freezer, depending on the sample normal habitat temperature range, 4 C, 20 C, 80 C (see step 1b) N/A N/A (Continued on next page) ll OPEN ACCESS STAR Protocols 3, 100968, March 18, 2022 3 Protocol Continued REAGENT or RESOURCE SOURCE IDENTIFIER Cell Sorter, here described BD Influx , or similar instruments: e.g., Wolf G2, that can do FACS into plates and tubes (steps 7 to 10) BD Biosciences nanocellect choose Wolf G2 2 sheath fluid tanks and one waste tank for the BD Influx Cell Sorter (steps 7 to 10) Order specific to instrument Order specific to instrument small sonic bath, e.g., Ultrasonic Model B200 (steps 7 to 10) VWR # 100491-732 small vortex, e.g., VWR LabDancer Mini Vortexer. (steps 7 to 31) VWR # 82019-172 PCR hood or Biosafety hood with UV bulbs, any (steps 1 to 32) N/A N/A Stratagene UV Stratalinker 2400 or equivalent (steps 1 to 31) LabX or ALT Stratagene Stratalinker 2400 or ALT any microscope with fluorescent light capability and glass slides (steps 1 to 10) N/A N/A BioRad SFX384 Real-time thermocycler or similar (step 11 to 31, step 1d) Bio-Rad Laboratories 12011452 Optional: Labcyte ECHO525 automatic liquid handler – optional for low volume reactions. (step 11 to 24) Labcyte.com 001-10080 Benchtop microcentrifuge, any for 1.5–2 mL tubes. (steps 11 to 36) Stellar Scientific BS-MFUG-12 Swing bucket centrifuge for plates, ideally refrigerated. (steps 11 to 36) Any Any Any regular thermocycler for 96-well plates. (step 32 to 36) Any Any ll OPEN ACCESS Protocol ..
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